Rat Anti-Mouse PDGFR-beta (Clone 4C54) mAb

Molecule:

PDGFRB

Synonyms:

Platelet-derived growth factor receptor beta, PDGFR-beta, CD140 antigen-like family member B, CD140b, Platelet-derived growth factor receptor 1, PDGFR-1

Species:

Mouse

Cat. No.:

CMP115 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$800.00 BUY

Description:

PDGFRα and Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF, and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the α and β receptors. Others have only the α receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the β receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages). Recombinant mouse and human soluble PDGF Rβ bind PDGF with high affinity and are potent PDGF antagonists.

Uniprot Name:

Platelet-derived growth factor receptor beta

Uniprot ID:

P05622

Data PDF:

CMP115

Specificity:

Recognizes mouse PDGFR-beta. No cross-reactivity with mouse PDGF receptor-alpha.

Host Species:

Rat

Immunogen:

Recombinant mouse PDGFR-beta extracellular domain

Isotype:

IgG2

Clone:

4C54

Formulation:

Lyophilized from sterile filtered PBS

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Application notes:

Western blot: suggested dilution 1:500-1:1000
IHC-P: suggested dilution 1:50-1:200

Storage/Stability:

Lyophilized antibody is stable for at least 1 year from date of receipt at -20°C to -80°C. Reconstituted antibody may be prepared as aliquots and stored at -20°C to -80°C for at least six months. Avoid repeated freeze-thaw cycles.