PDGFRA

Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, CD140 antigen-like family member A, CD140a

Mouse

CMP114 pdf (datasheet)

100 μg

$800.00 BUY

PDGFRα and Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF, and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the α and β receptors. Others have only the α receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the β receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages). Recombinant mouse and human soluble PDGF Rβ bind PDGF with high affinity and are potent PDGF antagonists.

Platelet-derived growth factor receptor alpha

P26618

CMP114

Recognizes mouse PDGFR alpha. No cross-reactivity to mouse PDGFR beta.

Rat

Recombinant Mouse PDGFR alpha extracellular domain

IgG2

3G28

Lyophilized from sterile filtered PBS

Protein G affinity chromatography

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Western blot: suggested dilution 1:400 - 1:800
IHC-P: suggested dilution 1:50 - 1:100

Lyophilized antibody is stable for at least 1 year from date of receipt at -20°C to -80°C. Reconstituted antibody may be prepared as aliquots and stored at -20°C to -80°C for at least six months. Avoid repeated freeze-thaw cycles.