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M6II-7 can be used to detect human MRP6 in cells and tissues. 1 ml > 200 tests.
ICC: recommended 1:20 - 1:50 dilution on acetone-fixed cytospin preparations.
IHC: recommended 1:20 - 1:50 on acetone-fixed frozen sections, followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). Un-reactive on standard formaldehyde-fixed paraffin-embedded material.
WB: recommended 1:20 - 1:50 dilution followed by anti-rat-HRP.
The optimal concentration should be determined by the user for each specific application.