Description:
Recombinant (Streptococcus stain G148) monomeric protein lacking cysteine residues;
Mr 22 kDa (Mr by SDS-PAGE - 32 kDa)
Contains a Streptavidin Binding Peptide (SBP) tag
Specificity:
Protein G binds the Fc portion of IgGs from several species including human and mouse (IgG1 weakly) subclasses. it does not bind IgA, IgE, IgM or serum albumin.
Formulation:
PBS, pH 7.4, 50% glycerol. No preservative added.
Application notes:
Working dilutions for ELISA 1:4,000 - 1:8,000. Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Storage/Stability:
2-8°C for short term storage. Store as working aliquots at -20°C to -80°C for long term. Avoid repeated freeze/thaw cycles.