Specificity:
Protein A binds the Fc portion of IgG1, IgG2, IgG3 (weakly), IgG4 and mouse IgG1 (weakly), IgG2a, IgG2b and IgG3; Protein A also binds monkey, rabbit, porcine, guinea pig, canine and feline IgG.
Application notes:
Working dilutions for ELISA 1:4,000 - 1:8,000. Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.