Normal Human Brain Tissue Lysate

Species:

Human

Cat. No.:

PX335 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$250.00 BUY

Description:

Human tissue lysates are ideal for biomarker identification and screening, antibody detection and characterization, protein expression and interaction studies, ligand binding. ELISA, immunoprecipitation, 1D and 2D gel electrophoresis and Western blotting. Tissues and cell lines are flash frozen within 5-10 minutes of removal. Widely accepted buffers are used to fractionate soluble (modified RIPA buffer) and insoluble (Urea-based buffer) proteins. All human materials are obtained under strict bioethical standards using IRB-approved protocols that ensure patient confidentiality, safety and informed consent.

Concentration:

2.0 mg/ml

Data PDF:

PX335

Source:

Human Brain

Formulation:

Tissue lysate is supplied frozen in modified RIPA buffer containing 1 mM EDTA, 1 mM PMSF, 0.1% Na deoxycholate, 0.1% SDS, 5 μg/ml aprotinin, 5 μg/ml leupeptin, and 5 μg/ml Pepstatin A. Centrifuge vial before use.

Storage/Stability:

Store at -80°C.

Application notes:

These lysates have not been subjected to heat denaturation or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets. For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.