Cat. No.:
CSI12857B
pdf (datasheet)
Description:
Streptavidin is a homotetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology because of its unique high affinity for biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association yet observed for noncovalent binding of a protein and small ligand in aqueous solution (Kassoc = 10-14 mol/L). The complexes are also extremely stable over a wide range of temperature and pH.
Concentration:
25 mg/ml prior to lyophilization
Uniprot Name:
Streptavidin
Source:
Bacterium Streptomyces avidinii
Formulation:
10 mM potassium phosphate buffer, pH 6.5
Biological Activity:
16.8 U/mg ( 1 U binds 1 µg Biotin)
Storage/Stability:
Store at -20 °C to -80 °C.
Application notes:
Streptavidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
