Native Porcine Glutamate Pyruvate Transaminase, GPT/ALT

Molecule:

GPT/ALT

Synonyms:

Alanine aminotransferase 1, ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--pyruvic transaminase 1, Glutamic--alanine transaminase 1, EC:2.6.1.2

Species:

Porcine

Cat. No.:

CSI14647B pdf (datasheet)

Quantity/Size:

25 KU

Description:

Glutamate Pyruvate Transaminase derived from Porcine Heart. Glutamate Pyruvate Transaminase (GPT) is an enzyme that catalyzes the reversible transfer of an amino group from alanine to alpha-ketoglutarate. GPT is found in serum and in various bodily tissues but is most commonly associated with the liver.

Concentration:

1.0 mg protein /mg (A280), lot specific
0.63 mg protein/mg (Coomassie)

Entrez Symbol:

GPT

Uniprot Name:

Alanine aminotransferase 1

Uniprot ID:

P13191

EC number:

2.6.1.2

Data PDF:

CSI14647x

Source:

Porcine Heart

Molecular Weight:

100 kDa

Formulation:

Lyophilized from Tris-chloride buffer, pH 7.0 with sodium chloride, EDTA , DTT, alpha-ketoglutarate and pyridoxal-5-phosphate

Biological Activity:

110 U/mg (DimensionTM Clinical Chemistry System), lot specific

Specific Activity:

110 U/mg protein (A280), lot specific
180 U/mg protein (Coomassie)

Storage/Stability:

Store at -20°C to -80°C up to 1 year

Application notes:

One unit will catalyze the transamination of one micromole per minute of L-alanine to alpha-ketoglutarate, forming L-glutamate and pyruvate at 37°C and pH 7.4.