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Native Porcine Creatine Kinase

Molecule:

Creatine kinase

Synonyms:

Creatine kinase M-type, Creatine kinase B-type, Creatine Phosphokinase, CPK

Species:

Porcine

Cat. No.:

CSI19647B pdf (datasheet)

Quantity/Size:

250 KU

Price:

$1,600.00 BUY

Description:

Creatine kinase (CPK) is an enzyme that consists of two subunits, which can be either B (brain type) or M (muscle type). Three different isoenzymes exist: CKBB, CKMM, and CKMB. This enzyme expressed by various tissues and cell types. Heart muscle expresses CKMM at 70%, CKMB at 25-30%. CPK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine (PCr) and adenosine diphosphate (ADP). This CPK enzyme reaction is reversible, so that also ATP can be generated from PCr and ADP. Creatine kinase's clinical significance: detection of heart disease, liver disease, diseases of the central nervous system and thyroid disease.

Entrez Symbol:

CKM, CKB

Uniprot ID:

Q5XLD3 M-type, Q29594 B-type

Data PDF:

CSI19647

Source:

Porcine Heart

Formulation:

Lyophilized from sodium acetate, 1 mM DTT, 1.5 mM EDTA, pH 7.0

Biological Activity:

One Unit will transfer 1 mmole of phosphate from Creatine Phosphate to ADP per minute at pH 7.4 and 37°C.

Specific Activity:

> 300 U/mg (Dimension® Clinical Chemistry System)

Reconstitution:

Reconstitute in deionized water or dilute buffer.

Storage/Stability:

Store as supplied at -20°C to -80°C for at least 1 year.