Native Porcine Calpain-1, Erythrocytes

Synonyms:

Calpain-1 catalytic subunit and Calpain small subunit 1 (Calpain regulatory subunit)

Species:

Porcine

Cat. No.:

CNP006B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$900.00 BUY

Description:

Calpain-1 is a calcium activated neutral cysteine protease. It is a heterodimer of a Catalytic 80 kDa subunit and a 30 kDa subunit, which requires 50 μM Ca2+ for half-maximal activity in vitro. Its participation in events such as cell division, signal transduction, and long term potentiation has been suggested. Calpains have also been proposed to play a role in various pathological processes associated with altered metabolism and altered calcium homeostasis.

Entrez Symbol:

CAPN1, CPNS1

Uniprot Name:

Calpain-1 catalytic subunit, Calpain small subunit 1

Uniprot ID:

P35750, P04574

Data PDF:

CNP006

Molecular Weight:

110 kDa (80 kDa catalytic subunit + 30 kDa regulatory subunit)

Formulation:

20 mM imidazole-HCl, 5mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8

Purity:

≥ 95% by SDS-PAGE

Biological Activity:

> 1000 Units/mg
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25°C using the Calpain Activity Assay Kit, Fluorogenic (EMD Cat. No, QIA120). Note: one caseinolytic unit=9 fluorogenic units.

Storage/Stability:

Store at -80°C up to 1 year