Synonyms:
Urokinase-type plasminogen activator, U-plasminogen activator, uPA
Description:
Urokinase Plasminogen Activator (uPA) is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts Plasminogen to Plasmin by specific cleavage of an Arg-Val bond in Plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal Bchain. This two-chain derivative is also called HMW-uPA (High Molecular Weight uPA). HMW-uPA can be further processed into LMW-uPA (Low Molecular Weight uPA) by cleavage of chain A into a short chain A (A1) and an ATF-uPA (Amino-Terminal Fragment uPA). LMW-uPA is proteolytically active but does not bind to the uPA Receptor (uPAR).
Uniprot Name:
Urokinase-type plasminogen activator
Molecular Weight:
54.0 kDa (411 aa) dimer
Formulation:
Lyophilized from a sterile-filtered 1 mg/ml solution containing phosphate buffer
Biological Activity:
1 nM Human uPA will cause a change in absorbance at 405nm of 0.001/minute at room temperature in 100 µl 50 mM Tris-HCl, 100 mM NaCl, pH 7.4, using 0.6 mM S2444 as the substrate.
Specific Activity:
> 68,000 IU/mg, lot specific
Reconstitution:
Centrifuge vial prior to opening. Reconstitute with sterile water to a concentration of at least 0.1 mg/ml. After complete solubilization of the protein, it may be further diluted to other aqueous solutions.
Storage/Stability:
Lyophilized product is stable at room temperature for shipping purpose. On receipt, store below -20°C. After reconstitution, store for up to 1 week at 2-8°C or in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
