Native Human Neutrophil Elastase Lyophilized

Molecule:

ELANE

Synonyms:

ELA-2, GE, NE, HLE, HNE, ELA2, SCN1, PMN-E

Species:

Human

Cat. No.:

CRN115CL pdf (datasheet)

Quantity/Size:

5 mg

Price:

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Description:

Chromatographically purified from human leucocytes and lyophilized. The protein is greater than 95 percent pure by SDS-PAGE and is free of cathepsin G, MPO and lysozyme. 800-900 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA and 15000-19000 units per mg protein on the substrate MeO-Suc-Ala-Ala-Pro-Val-pNA. One unit will hydrolyze 1 umole of substrate per minute at pH 7.5 and 25 °C. Proteolysis can be inhibited by benzamidine. Autolysis and proteolysis can be inhibited by elastatinal. However, elastatinal is also an inhibitor of elastase. Low pH protects the elastase from both autolysis and proteolysis.

Uniprot Name:

Neutrophil elastase

Uniprot ID:

P08246

Data PDF:

CRN115CL

Source:

Human Neutrophils

Formulation:

Lyophilized in acetate buffer

Purity:

≥96% by SDS-PAGE

Specific Activity:

Lot specific, measured as one unit will hydrolyze one micromole of Methoxy succinylalanine-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5.

Reconstitution:

Recommend reconstitution at ≥ 1 mg/mL in a buffer such as Tris-buffered saline, pH 7.5. Further dilutions should include a carrier such as 1% BSA.

Storage/Stability:

Upon receipt, store desiccated at 2-4°C for up to one year. Upon reconstitution, store in working aliquots between -20 to -80°C. Avoid repeated freeze-thaw cycles.