Native Human Myeloma IgE, Partially Purified

Molecule:

IgE

Synonyms:

Immunoglobulin heavy constant epsilon, Immunoglobulin E

Species:

Human

Cat. No.:

CNH024 pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$950.00 BUY

Description:

This product is Human IgE, produced in cell culture from a IgE secreting human myeloma cell line. This IgE product has advantages of less lot-to-lot variation, more uniform product (monoclonal vs. polyclonal) than IgE from human plasma. It is partially purified, with any remaining contaminants from the FBS used in cell culture.

IgE is the least abundant immunoglobulin in plasma, found at only 0.05% of the Ig concentration, compared to 75% for the IgGs at 10 mg/ml. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a human myeloma cell line is a useful protein for studying immunoglobulin behavior. The affinity purified IgE reacted only with anti IgE and not with anti IgG, IgA, IgM or IgD by immunodiffusion and IEP techniques.

Concentration:

> 5.0 mg/ml, by Cobas IgE, lot specific

Uniprot Name:

Immunoglobulin heavy constant epsilon

Uniprot ID:

P01854

Data PDF:

CNH024

Source:

Human

Molecular Weight:

200 kDa

Formulation:

100 mM Tris-HCl, 200 mM NaCl, pH 7.5

Purity:

Partially purified from cell culture supernatant with residual impurities from fetal bovine serum used in cell culture.

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.