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Native Human Glutamate Oxaloacetate Transaminase is derived from Liver. Two GOT isoenzymes are present in humans. They have high similarity. GOT1, the cytosolic isoenzyme, derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.
>1 U/mg protein, lot specificOne unit will catalyze the transamination of one micromole of L-aspartate to αketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8. Measured at 340 nm as one equimolar amount of NAD produced
CK: < 1.0% ALT/GPT: < 2.0% LDH: < 10%Ammonia: < 0.01 micromole/mg