Complement C3b, Complement component 3b

Human

CSI19503 pdf (datasheet)

250 μg

$550.00 BUY

C3b is derived from native C3 upon cleavage and release of C3a. It is prepared by cleavage with the alternative pathway C3 convertase. This is important because only a single bond in C3 is cleaved by the native convertase, while cleavage by other proteases, such as trypsin, results in multiple cleavages at many other sites in the protein. Native human C3b is a glycosylated (~2.8%) polypeptide containing two disulfide-linked chains. C3b is central to the function of all three pathways of complement. Initiation of each pathway generates proteolytic enzyme complexes (C3 convertases) which are bound to the target surface. These enzymes cleave a peptide bond in C3 releasing the anaphylatoxin C3a and activating C3b. For a brief time (~60 µs) this nascent C3b is capable of reacting with and covalently coupling to hydroxyl groups on the target surface. Carbohydrates are the favored target, but protein hydroxyls and amino groups also react. This process of tagging the target surface with C3b is called opsonization.

1.0 mg/ml, lot specific

Complement C3

P01024

CSI19503

Human Serum

176 kDa, two disulfide-linked subunits

Sterile-filtered PBS, pH 7.2

>95% by SDS-PAGE, trace IgG, IgA, IgM, albumin, C5, Factor B, Factor H, Factor I by immunochemistry.

Store as supplied at -80°C for up to 1 year.