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Native Human Calpain-1, Erythrocytes

Synonyms:

Calpain-1 catalytic subunit, Calpain-1 small subunit,

Species:

Human

Cat. No.:

CRC118B pdf (datasheet)

Quantity/Size:

500 µg

Price:

$1,350.00 BUY

Description:

Calpain-1 (μ-Calpain) is a calcium activated neutral cysteine protease. It is a heterodimer of a Catalytic 80 kDa subunit and a 30 kDa subunit, which requires 50 μM Ca2+ for half-maximal activity in vitro. Its participation in events such as cell division, signal transduction, and long term potentiation has been suggested. Calpains have also been proposed to play a role in various pathological processes associated with altered metabolism and altered calcium homeostasis.

Entrez Symbol:

CAPN1, CAPNS1

Uniprot Name:

Calpain-1 catalytic subunit, Calpain-1 small subunit,

Uniprot ID:

P07384 catalytic subunit

P04632 small (regulatory) subunit

Data PDF:

CRC118

Source:

Human erythrocytes

Molecular Weight:

110 kDa, whole molecule

80 kDa catalytic subunit

30 kDa small (regulatory) subunit

Formulation:

20 mM imidazole, 5 mM beta-mercaptoethanol, 1 mM EDTA , 1 mM EGTA, 30% glycerol, pH 6.8.

Purity:

> 95% as determined by SDS-PAGE.

Biological Activity:

One unit is defined as the amount of enzyme that will hydrolyze one ρmol Suc-LLVY-AMC per minute at 25°C using the Calpain Activity Assay Kit, Fluorogenic (EMD Cat. No. QIA120)

Specific Activity:

> 1,000 units/mg protein

Storage/Stability:

Store at -80oC for up to 1 year or in working aliquots at -80oC. Avoid repeated freeze-thaw cycles.