Native Bovine Thrombin, Immobilized

Synonyms:

Prothrombin, Coagulation factor II, F2

Species:

Bovine

Cat. No.:

CRU131A pdf (datasheet)

Quantity/Size:

2 ml of packed resin

Price:

$2,500.00 BUY

Description:

Immobilized Bovine Thrombin is ideal for cleavage of recombinant fusion proteins with an Leu-Val-Pro-Arg/Gly-Ser thrombin recognition sequence. The agarose resin is easily removed by centrifugation or filtration and is reusable.

Concentration:

2.0 mg protein/ml resin, lot specific

Entrez Symbol:

F2

Uniprot Name:

Prothrombin

Uniprot ID:

P00735

Data PDF:

CRU131

Source:

Bovine plasma

Formulation:

1:1 slurry of resin in 0.1 M Tris, 0.15 M NaCl, 0.02% sodium azide, pH 7.5

Purity:

> 95% by SDS-PAGE

Biological Activity:

Immobilized Thrombin Digestion of Protein

Storage/Stability:

Store at 2-8°C for up to 1 year.

Application notes:

Optimization of immobilized thrombin protein digestion protocol is required for specific applications. Recommended reaction conditions are pH 7.0 to 9.0 at 37°C. The reaction rate will be increased by increasing the enzyme to protein substrate ratio and incubation temperature. For example, a typical digestion of 4 hours to overnight can be achieved using a ratio of 1:25 enzyme to protein substrate, while it is recommended to use 1:10 enzyme to protein substrate for accelerated thrombin digestion (30-60 minutes).