• Home /
  • Native Bovine Insulin, Cell Culture Grade

Native Bovine Insulin, Cell Culture Grade

Species:

Bovine

Cat. No.:

CRI116B pdf (datasheet)

Quantity/Size:

100 mg

Price:

$800.00 BUY

Description:

Insulin is a two-chain polypeptide hormone produced in the pancreatic β cells and it is the most studied hormone. Insulin regulates glucose uptake into muscle and fat cells by recruiting membrane glucose transporter Glut-4 to cell surface.

The amino acid sequence of insulin is extremely well conserved. Bovine insulin differs from human insulin in only three amino acid residues, similar enough to be clinically effective in humans. Bovine Insulin is used as growth supplement in culturing cells at the concentration ranging from 1 to 10 micrograms per milliliter of medium.

Entrez Symbol:

INS

Uniprot Name:

Insulin

Uniprot ID:

P01317

Data PDF:

CRI116

Source:

Bovine Pancreas

Molecular Weight:

5.8 kDa

Physical State:

Lyophilized

Biological Activity:

Potency ≥25 USP units/mg by HPLC

Reconstitution:

Insulin has low solubility at neutral pH. It can be solubilized at 2 mg/ml in dilute acetic or hydrochloric acid, pH 2-3. Prepare stock solution at 10 mg/ml with acidified water, pH 2.0 (use 0.1 ml acetic acid or HCL to prepare pH 2 water.) Sterile filter and apportion into working aliquots.

Storage/Stability:

Store at -20°C to -80°C

Application notes:

Suggested Usage: 1 to 10 μg/ml bovine insulin in medium
Cell Culture tested: 5 μg/ml of bovine insulin support robust growth of human keratinocytes