Description:
Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth
promoting factors for vascular endothelial cells of mammalian origin. ECGF has also
been reported to be beneficial as a media supplement for the fusion and growth of
hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is
prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a
sterile solution containing NaCl and streptomycin sulfate. Endothelial cells from human
umbilical vein (HUVEC) can be established as primary cultures by traditional methods.
The serial propagation of these cells has proved to be difficult. The long-term propagation
of these cells in vitro can be achieved with an extract prepared from porcine brain. The
introduction of a fibronectin or collagen matrix to the cell culture system allows to
cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be
reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF.
ECGF has also been reported to eliminate the need for feeder cells in the clonal growth
of hybridomas and other cell types.
Uniprot Name:
Heparin-binding growth factor 1
Formulation:
Lyophilized from a sterile filtered solution in H2O without preservative.
Biological Activity:
The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal.
Storage/Stability:
Store as supplied at -20°C to -80°C
Application notes:
Bovine ECGF is effective on Mouse, Bovine and Human cells. Optimum concentration for HUVEC ranges from 50-200 µg/mL, optimal concentration with heparin (50 µg/mL) is about 10 µg/mL. As a growth supplement for use in monoclonal antibody production, the optimum range is 25 to 100 µg/mL.
