Description:
The Cell Sciences® Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF in serum, plasma
and cell culture supernatants. This assay employs an antibody specific for mouse VEGF coated on a 96-
well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to
the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse VEGF antibody
is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to
the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops
in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and
the intensity of the color is measured at 450 nm.
