The Mouse MPO (Myeloperoxidase) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MPO in plasma (serum is not recommended in this assay due to mouse MPO being released from neutrophils into serum in the process of blood coagulation) and cell culture supernatants. This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Specifications:
- Specificity: Mouse Myeloperoxidase (MPO)
- Sensitivity: 600 pg/ml
- Range: 0.61 to 150 ng/ml
- Sample Type: Cell supernatants, serum, plasma samples.
Reagent included:
- Microplate coated with Anti-Mouse MPO
- Wash Buffer Concentrate (20x)
- Recombinant Mouse MPO Standards
- Assay Diluent C: Standard/Sample - Plasma Diluent Buffer
- Assay Diluent B (5x): Standard/Sample/Cell Culture Medium Diluent
- Detection Antibody: Anti-Mouse MPO, Biotinylated
- Streptavidin-HRP Concentrate (400x)
- TMB One-Step Substrate Reagent (3, 3’, 5, 5’-tetramethylbenzidine in buffered solution)
- Stop Solution (0.2 M Sulfuric Acid)
