Mouse Anti-Human Vimentin (Clone V9) mAb

Species:

Human

Cat. No.:

MON3005-5 pdf (datasheet)

Quantity/Size:

5 ml

Concentration:

approximately 30 ug/ml

Description:

Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57 kD. Immunohistochemical staining for Vimentin is thus character¬istic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Entrez Symbol:

VIM

Uniprot ID:

P08670

Data PDF:

MON3005

Specificity:

This antibody stains the 57 kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. It does not stain mouse or rat vimentin.

Host Species:

Mouse

Immunogen:

vimentin isolated from cow lens

Isotype:

IgG1

Clone:

V9

Formulation:

Purified cell culture supernatant in PBS containing 1% BSA, 20 mM sodium azide

Application:

IHC-F, IHC-P, WB

Application notes:

IHC-F: suggested dilution 1:300

IHC-P: suggested dilution 1:50-1:100

Storage/Stability:

Store at 2-8°C for up to 1 year.