Mouse Anti-human VEGFR-1 (Clone 8F12) Agonistic mAb

Synonyms:

Vascular endothelial growth factor receptor 1, VEGFR-1, Fms-like tyrosine kinase 1, FLT -1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor

Species:

Human

Cat. No.:

CMV125 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$1,100.00 BUY

Description:

Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The antibody will bind near the ligand binding site of the receptor and has agonistic activity.

Uniprot Name:

Vascular endothelial growth factor receptor 1

Uniprot ID:

P17948

Data PDF:

CMV125

Specificity:

Human VEGFR1, no reactivity with hVEGFR2

Host Species:

Mouse

Immunogen:

Human VEGFR1 N-terminal fragment (aa 25-250)

Isotype:

IgG1

Clone:

8F12

Formulation:

Lyophilized from sterile filtered PBS

Purification:

Protein G Chromatography

Reconstitution:

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Application notes:

Western Blot
VEGFR1 activation: Induces hVEGFR1 phosphorylation in HUVECs at > 2.0 µg/ml.

Storage/Stability:

Lyophilized antibody is stable for at least 1 year at -20°C to -80°C. Reconstituted antibody is stable for six months in working aliquots at -20°C to-80°C. Avoid repeated freeze-thaw cycles.