The antibody reacts with the extra-cellular part of the TNF-RI. It also reacts with the soluble receptor. TNF-RI is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-α. Induction of cytotoxicity and other functions are mediated largely via TNF-RI.

The antibody is an antagonistic antibody useful for cell culture experiments, flow cytometry and histology on frozen sections. Furthermore the antibody can be used for immunoprecipitation, Western blot and immunoassays.
Advised positive control: - immunohistochemistry: human lymphnodes; - flow cytometry: Hela cells.