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Mouse Anti-Human TIMP-1 (Clone 4A16) mAb

Molecule:

TIMP1

Synonyms:

Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Tissue Inhibitor of Metalloproteinase 1, TIMP-1

Species:

Human

Cat. No.:

CMT150 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$1,000.00 BUY

Description:

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Uniprot Name:

Metalloproteinase inhibitor 1

Uniprot ID:

P01033

Data PDF:

CMT150

Specificity:

Recognizes human TIMP-1

Host Species:

Mouse

Immunogen:

Recombinant human TIMP1

Isotype:

IgG1

Clone:

4A16

Formulation:

Lyophilized from sterile-filtered PBS

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Application:

IHC, WB

Application notes:

Western blot: suggested dilution 1:100-1000
IHC-P: suggested dilution 1:50-200
ELISA: as the capture Ab paired with polyclonal anti-hTIMP-1

Storage/Stability:

Lyophilized antibody is stable for 1 year at -20°C to -80°C. Reconstituted antibody is stable for six months in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.