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Mouse Anti-Human PDGF-AA (Clone 6D35) mAb

Molecule:

PDGF-AA

Synonyms:

Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF-AA

Species:

Human

Cat. No.:

CMP106 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$1,090.00 BUY

Description:

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human PDGF-AA recombinant protein. Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the αgranules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β.

Entrez Symbol:

PDGFA

Uniprot Name:

Platelet-derived growth factor subunit A

Uniprot ID:

P04085

Data PDF:

CMP106

Specificity:

Recognizes human PDGF-AA, homodimer

Host Species:

Mouse

Immunogen:

Recombinant human PDGF-AA, homodimer

Isotype:

IgG2

Clone:

6D35

Formulation:

Lyophilized from sterile-filtered PBS

Purification:

Protein G Chromatography

Reconstitution:

Centrifuge vial prior to opening. Reconstitute with 500 µl sterile PBS for a concentration of 200 µg/ml

Application notes:

Western Blot: suggested dilution 1:1,000-1:2,000
IHC: suggested dilution 1:50- 1:200

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store lyophilized antibody at -80°C for at least 1 year. Reconstituted antibody is stable for at least six months in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.