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  • Mouse Anti-Human Neurofilament Light and Heavy (Clone 2F11) mAb

Mouse Anti-Human Neurofilament Light and Heavy (Clone 2F11) mAb

Molecule:

NF

Synonyms:

Neurofilament light polypeptide, 68 kDa neurofilament proteinNeurofilament heavy polypeptide, 200 kDa neurofilament protein

Species:

Human

Cat. No.:

MON3004-1 pdf (datasheet)

Quantity/Size:

1 ml

Price:

$490.00 BUY

Concentration:

15 µg/mL IgG1, anti-Neurofilament

Description:

The monoclonal antibody recognizes human neurofilaments. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.

Entrez Symbol:

NEFL, NEFH

Uniprot Name:

Neurofilament light polypeptide, Neurofilament heavy polypeptide

Uniprot ID:

P07196 light, P12036 heavy

Data PDF:

MON3004

Specificity:

This antibody stains the 70 kD (light) and 200 kD (heavy) polypeptides of neurofilament.

Host Species:

Mouse

Immunogen:

Neurofilament from human brain

Isotype:

IgG1

Clone:

2F11

Conjugate:

Unconjugated

Formulation:

Cell culture supernatant diluted in 0.15 M PBS, 1% BSA, 0.1% sodium azide.

Application:

IHC, WB

Application notes:

The antibody stains neurons in sections of brain and other tissues. Frozen sections show excellent staining. On sections from formalin fixed and paraffin embedded tissue the staining is somewhat reduced.

IHC - frozen: dilute approximately 1:160, preferably in PBS
IHC - paraffin: dilute approximately 1:50.
WB: dilute 1:20 (lesser amounts of the antibody may be sufficient)

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store antibody at 2-8°C for up to 1 year.