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Mouse Anti-Human CXCL9 / MIG mAb

Molecule:

MIG

Synonyms:

Chemokine (C-X-C motif) Ligand 9, Monokine Induced by Gamma Interferon, MIG, CMK, Humig, SCYB9, crg-10

Species:

Human

Cat. No.:

CMM104 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$700.00 BUY

Description:

The mouse monoclonal antibody recognizes Human CXCL9/ MIG, a small cytokine belonging to the CXC chemokine family. CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma and functions by interacting with the Chemokine (C-X-C motif) Receptor 3 (CXCR3).

Entrez Symbol:

CXCL9

Uniprot Name:

C-X-C motif chemokine 9

Uniprot ID:

Q07325

Data PDF:

CMM104

Specificity:

Recognizes Human CXCL9

Host Species:

Mouse

Immunogen:

Recombinant Human MIG (CXCL9), 12.2 kDa protein

Isotype:

IgG1K

Clone:

87M-101

Formulation:

Lyophilized from a sterile filtered carrier-free solution

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/mL.

Application notes:

Sandwich ELISA: recommended concentration of 0.125 - 0.25 µg/mL (100 µl/well) will detect at least 100 pg/mL of recombinant human CXCL9/MIG when used with an appropriate biotinylated detection antibody at a concentration of approximately 0.5 - 1µg/mL.

Western Blot: recommended concentration of 0.5 - 2.0 µg/mL, when used in conjunction with compatible secondary reagents, will detect a minimum of 0.25 - 0.5 ng/lane (non-reducing conditions) and 2.0 - 4.0 ng/lane (reducing conditions) of recombinant human CXCL9/MIG.

Storage/Stability:

Lyophilized antibody is stable for up to 1 month at room temperature. Upon receipt, store below -20°C. After reconstitution, antibody may be store at 2-8°C for up to two weeks or in working aliquots at -20°C to -80°C for at least six months. Avoid repeated freezethaw cycles.