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  • Mouse Anti-Human CD3 (Clone CLB-T3/4.E, 1XE) mAb

Mouse Anti-Human CD3 (Clone CLB-T3/4.E, 1XE) mAb

Molecule:

CD3

Synonyms:

T3 antigen, T-cell surface glycoprotein CD3

Species:

Human

Cat. No.:

M1654A pdf (datasheet)

Quantity/Size:

1 mg

Price:

$2,700.00 BUY

Concentration:

~1.0 mg/ml, lot specific

Description:

The monoclonal antibody is intended to be used to induce the proliferation of resting T lymphocytes. In general, two signals are required to activate T lymphocytes into proliferation. In vitro, both signals can be given by the proper combination of monoclonal antibodies. The CD3 molecule (alpha, beta, gamma and zeta chains) exists in a complex with the T cell receptor (TCR). By binding CD3 this antibody mimics the signal through the TCR:the "first signal." The binding of anti-CD28 mAbs to T cells was found to enhance stimulation of the cells by anti-CD3 mAbs. Therefore, CD28 is regarded as a co-stimulatory molecule, providing the "second signal."

Data PDF:

M1654

Specificity:

Recognizes human CD3 antigen (T3-antigen), which is expressed on human T lymphocytes. It reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes.

Host Species:

Mouse

Immunogen:

Human T lymphocytes

Clone:

CLB-T3/4.E, 1XE
This clone is a switch variant from the original clone 1X1.

Formulation:

Culture supernatant is concentrated to an antibody level comparable to ascites fluid.

Application:

Functional assay

Application notes:

To induce the proliferation of resting T lymphocytes for further study. In general, two signals are required to activate T lymphocytes into proliferation. In vitro, both signals can be given by the proper combination of monoclonal antibodies, in this respect, monoclonal antibodies against CD2, CD3 and CD28 have provided much information on the stimulatory mechanism. It was found that anti-CD2 antibodies are also able to stimulate T cells, although only in the presence of a second signal, which can be given either by more anti-CD2 antibodies directed against other epitopes on the CD2 molecule, and / or e.g. by an anti-CD28 antibody. The binding of anti-CD28 McAbs to T cells was found to enhance stimulation of the cells by anti-CD2 and anti-CD3 McAbs. Therefore, CD28 is regarded as a ‘co-stimulatory’ molecule.

Storage/Stability:

Store in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.