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Mouse Anti-Human CCL20 / MIP-3 alpha mAb

Molecule:

MIP-3 alpha/CCL20

Synonyms:

C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Small-inducible cytokine A20

Species:

Human

Cat. No.:

CMM106 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$700.00 BUY

Description:

The mouse monoclonal antibody recognizes human CCL20 also called MIP-3 alpha, an antimicrobial protein in the subfamily of small cytokine CC proteins which are involved in immuno-regulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL20 signals through the CCR6 receptor. It is chemotactic for lymphocytes and dendritic cells, promotes adhesion of memory CD4+ T cells and can repress proliferation of myeloid progenitors. Human CCL20 is expressed in liver, lymph nodes, appendix, peripheral blood lymphocytes and lung.

Entrez Symbol:

CCL20

Uniprot Name:

C-C motif chemokine 20

Uniprot ID:

P78556

Data PDF:

CMM106

Specificity:

Recognizes human MIP-3 alpha

Host Species:

Mouse

Immunogen:

Recombinant human MIP-3 alpha

Isotype:

IgG1K

Formulation:

Lyophilized from PBS

Purification:

Protein G chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.

Application:

ELISA, WB

Application notes:

ELISA (sandwich): Recommended concentration of 2-4 μg/ml (100 μl/well) will detect at least 2000 pg/ml of rh MIP-3 alpha, when used with biotinylated antigen affinity purified anti-human MIP-3 alpha as the detection antibody at a concentration of approximately 1 -2 μg/ml.

WB: Recommended concentration of 0.2-0.4 μg/ml. The lower detection limit is 0.25 - 0.5 ng rh MIP-3 alpha/ lane under non-reducing conditions when used with compatible secondary reagents.

Storage/Stability:

Upon receipt, store at -20°C to -80°C. Reconstituted antibody is stable for at least 2 weeks at 2-8°C or as aliquots for at least 6 months when stored at -20°C to -80°C. Avoid repeated freeze-thaw cycles.