Mouse Anti-Human CCL19 / MIP3 beta (Clone G5_4E11.3) mAb

Molecule:

CCL19/MIP3 beta

Synonyms:

C-C motif chemokine 19, Beta-chemokine exodus-3, CK beta-11, Epstein-Barr virusinduced molecule 1 ligand chemokine, EBI1 ligand chemokine, ELC, Macrophage inflammatory protein 3 beta, MIP-3-beta, Small-inducible cytokine A19

Species:

Human

Cat. No.:

CMM108 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$700.00 BUY

Description:

The mouse monoclonal antibody recognizes human CCL19/MIP-1 beta, one of several CC cytokines, a family of secreted proteins involved in immunoregulatory and inflammatory processes, characterized by two adjacent cysteines. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Uniprot Name:

C-C motif chemokine 19

Uniprot ID:

Q99731

Data PDF:

CMM108

Specificity:

Recognizes human MIP3 beta

Host Species:

Mouse

Immunogen:

Recombinant human MIP-3 beta

Isotype:

IgG1K

Clone:

G5_4E11.3

Formulation:

Lyophilized

Purification:

Protein A chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.

Application notes:

ELISA: Recommended concentration 2-4 µg/ml (100 µl/well). In conjunction with a suitable detection antibody, CMM108 will detect at least 2 ng/ml of rh MIP-3 beta.

WB: Recommended concentration 1-2 µg/ml. In conjuction with compatible secondary reagent, the detection limit for rh MIP-3 beta is 2-4 ng/lane, under non-reducing conditions.

IHC: Recommended concentration 5 µg/ml, one hour at room temperature. In conjunction with suitable fluorescently-labeled secondary antibody, CMM108 stained PBMC.

Storage/Stability:

Lyophilized antibody is stable at room temperature for 1 month. For retention of full activity, store desiccated at -20°C for up to 1 year. Reconstituted antibody is stable for at least 2 weeks at 2-8°C, or for at least 6 months frozen in aliquots at -20 to -80°C. Avoid repeated freeze-thaw cycles.