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The antibody is directed to an immune dominant epitope (location unknown) of the Chlamydia LPS, which is a genus specific antigen. Using a DOT-EIA the antibody reacts strongly with following prototype strains:
- C. trachomatis: A B Ba C D E F G H I J K L L1 L2 L3 and mouse pneumonitis- C. psittaci : an ornithosis strain and a cat conjunctivitis strain- C. pneumoniae : TW183
IHC-P The monochonal antibody stains Chlamydia inclusions both in formalin-fixed tissue biopts embedded in paraffin, and in Hela-229 cells fixed in absolute methanol.
For staining fixed cells or tissue sections a dilution of 1:20 in PBS is advised.