Albumin

Mouse

CS376A pdf (datasheet)

1 kit

$760.00 BUY

Background: Albumin is a water-soluble protein with considerable structural stability, which makes up 60% of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions and medicinal products.

Assay Principle: Mouse albumin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-mouse albumin antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse albumin. Color development is proportional to the concentration of albumin in the samples.

Specifications:

• Sensitivity: 0.09 ng/ml
• Specificity: Mouse albumin (Does not distinguish IgG subclasses.)
• Range: 0.2-200 ng/ml
• Sample Type: Mouse serum, plasma, urine and other biological fluids. For research use only.
• Cross-Reactivity: Pooled normal plasma from rat was assayed and slight cross-reactivity was observed. Pooled normal plasma from rabbit, dog, pig, sheep cynomolgus monkey and human was assayed and no significant cross-reactivity was observed.

Kit Contents

• 96-well microtiter strip plate coated with anti-Mouse albumin antibody, blocked and dried on well surface
• 10X Wash Buffer Concentrate
• 5X Diluent
• Mouse albumin standard, lyophilized
• Anti-Mouse albumin primary antibody, concentrated polyclonal Ab
• TMB substrate solution

CS376A

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.