C3

AHUS5 , ARMD9 , ASPa , C3b , CPAMD1 , HEL-S-62p , C3

Human

CS409A pdf (datasheet)

1 kit

$800.00 BUY

Background: Complement Component 3 (C3), the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation. C3 is proteolytically activated by C3-convertase to the anaphylatoxin C3a and the opsonizing agent C3b. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus.

Assay Principle: C3 in normal human plasma ranges from 0.9-1.9 mg/ml with an average concentration of 1.39 mg/ml. The assay measures total human C3 in the 0.02-10 ng/ml range. Human C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human C3 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of C3 in the samples. A standard calibration curve is prepared using dilutions of purified C3 and is measured along with the test samples.

Specifications:

• Sensitivity: 0.0148 ng/ml
• Specificity: Human Complement C3
• Range: 0.02 - 10 ng/ml
• Sample Type: Human serum, plasma, urine, milk, saliva and cell culture samples.
• Cross-Reactivity: Pooled normal plasma from mouse, rat, pig, sheep, and horse were assayed and no significant cross-reactivity was observed.

Reagents Provided:

• 96-well microtiter strip plate coated with anti-Human C3 antibody
• Wash buffer concentrate
• Human C3 standard
• Anti-Human C3 primary antibody
• Horseradish peroxidase-conjugated Streptavidin
• TMB substrate solution

CS409A

Precoated plates

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.