Synonyms:
human colon carcinoma cells (SW480), human colorectal cancer cells (SW480)
Description:
Human SW480 Cell Lysate was prepared by homogenization in modified RIPA buffer (50
mM Tris-HCl, pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium
dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Cell debris was removed
by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Formulation:
Supplied as whole cell lysate in 1 x SDS sample buffer (50 mM Tris-HCL, pH 6.8 + 12.5% glycerol + 1% SDS + 0.01% bromophenol blue) containing 5% βmercaptoethanol.
Storage/Stability:
Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.
Application notes:
Centrifuge vial before use. Ready to load on SDS-PAGE for Western Blot. It is recommended to load 10 µg to 20 µg per lane for mini gel.
