Human

PX343A pdf (datasheet)

100 μg

$290.00 BUY

Human spleen tissue lysate was prepared by homogenization in a modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris were removed by centrifugation. Protein concentration was determined with BioRad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

2.0 mg/ml

PX343

Human

Supplied as whole cell lysate in 1 x SDS sample buffer containing 50 mM DTT.

Store at 2-8˚C for continuous use. Store as working aliquots at -80˚C for up to 1 year.

Centrifuge vial prior to use. Human spleen tissue lysate is ready to load on SDS - PAGE for Western blotting, 20 µg per lane is recommended for mini gel. The optimal concentration should be determined for each specific application.

WB