Human

PX724 pdf (datasheet)

100 μg

$250.00 BUY

Cell lysate has not been subjected to heat denaturation or reducing conditions. This allows the cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and western blotting for the detection of specific protein targets.

1.0 mg/ml

PX724

Human

PBS, pH 7.4, 0.1% SDS, 0.25% Na deoxycholate, 1 mM EDTA, 1 mM Na3VO4, 1 mM NaF, 1 µg/ml Aprotinin, 1 µg/ml Pepstatin-A, 1 µg/ml Leupeptin, 1 mM PMSF

Store at -80°C for up to 1 year.

For use in 1D and 2D gel electrophoresis, western blot, the addition of a denaturing gel loading buffer with reducing agents may be required.

Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, cell lysate in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.

ELISA, RIA, WB