Human

PX340A pdf (datasheet)

100 μg

$290.00 BUY

Human Lung Tissue Lysate was prepared by homogenization in a modified RIPA buffer (150 mM Sodium Chloride, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM PMSF, 1% Triton X-100, 1% Sodium Deoxycholic Acid, 0.1% SDS, 5 μg/mL Aprotinin, 5 μg/mL Leupeptin.) Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.01% Bromophenol Blue) containing 50 mM DTT.

2.0 mg/ml

PX340

Supplied in SDS sample buffer containing 50 mM DTT.

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Centrifuge vial before use. Human Lung Tissue Lysate is ready to load on SDS -PAGE for Western blotting. 20 µg per lane is recommended for a mini gel. The optimal concentration should be determined for each specific application.

WB