Human Liver Tissue Lysate

Species:

Human

Cat. No.:

PX339A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$290.00 BUY

Description:

Normal Human liver tissue lysate was prepared by homogenization in a modified RIPA buffer (150 mM Sodium Chloride, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM PMSF, 1% Triton X-100, 1% Sodium Deoxycholic Acid, 0.1% SDS, 5 μg/mL Aprotinin, 5 μg/mL Leupeptin.) Tissue and cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% Glycerol, 1% SDS + 0.01% Bromophenol Blue), containing 50 mM DTT

Concentration:

2.0 mg/ml

Data PDF:

PX339

Source:

Human liver

Formulation:

1 X SDS sample buffer with 50 mM DTT

Application notes:

Centrifuge vial before use. Human Liver Tissue Lysate Normal is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is recommended for mini gel. The optimal concentration should be determined for each specific application.

Application:

WB