Human

PX319A pdf (datasheet)

100 μg

$290.00 BUY

K562 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The K562 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

2.0 mg/ml

PX319

Lysate is supplied in 1 x SDS sample buffer containing 5% beta-mercaptoethanol.

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Centrifuge vial before use. K562 cell lysate is ready to load on SDS-PAGE for Western blot, 10-20 µg/lane is recommended for mini gel. The optimal concentration should be determined by the user for each specific application.

WB