Human IL-1 beta ELISPOT Kit, Pre-coated Plates

Molecule:

IL-1 beta

Species:

Human

Cat. No.:

CDK123D pdf (datasheet)

Quantity/Size:

20 plates

Price:

$2,995.00 BUY

Description:

The Cell Sciences® ELISPOT assay is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation.

IL-1 is primarily an inflammatory cytokine. It belongs to a group of cytokines with overlapping biologic properties (TNF alpha and IL-6). IL-1, TNF and IL-6 share the ability to stimulate T and B lymphocytes, increase cell proliferation, and initiate or suppress gene expression for several proteins exerting their effects by binding to specific receptors. The interleukin-1 family contains three structurally related polypeptides. The first two are IL-1 alpha and IL-1 beta, each of which has a broad spectrum of both beneficial and harmful biologic actions, and the third is IL-1-receptor antagonist, which inhibits the activities of interleukin-1.

Reagents provided:

  • Pre-coated 96-well PVDF-bottom plates
  • Biotinylated Detection Antibody
  • Streptavidin-Alkaline Phosphatase Conjugate
  • Bovine Serum Albumin (BSA)
  • BCIP/NBT Substrate

Data PDF:

CDK123

Storage/Stability:

Store the kit reagents between 2-8°C. Immediately after use, remaining reagents should be returned to cold storage 2-8°C. The expiration date of the kit and reagents is stated on the kit box label. The expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated during handling.