Background: IgM is the first immunoglobulin produced in the immune response and is the third most abundant immunoglobulin in serum. IgM is a disulfide-linked 970kDa pentamer that activates complement and is responsible for red blood cell agglutination. Each monomer consists of two mu heavy chains and two kappa or lambda light chains.
Assay Principle: Human IgM will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgM antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human IgM. Color development is proportional to the concentration of IgM in the samples.
Specifications:
- Sensitivity: 0.12 ng/ml
- Specificity: Total Human IgM
- Range: 0.2-200 ng/ml
- Sample Type: Serum, plasma, cell culture supernatant, ascites or other biological fluids.
- Cross-Reactivity: Pooled normal plasma from Cynomolgus monkey, rabbit, pig, and horse were assayed and significant cross-reactivity was observed. Pooled normal plasma from mouse, rat, and sheep were assayed and no significant cross-reactivity was observed.
Reagents provided:
- 96-well microtiter strip plate(s) coated with anti-Human IgM antibody, blocked and dried on well surface
- Wash Buffer Concentrate (10x)
- Human IgM standard, lyophilized
- Anti-Human IgM horseradish peroxidase antibody, lyophilized polyclonal antibody
- TMB substrate solution
