Human

CS222A pdf (datasheet)

1 x 96 tests each

$490.00 BUY

Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Assay Principle: Human IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human IgG. Color development is proportional to the concentration of IgG in the samples.

Specifications:

• Sensitivity: 0.084 ng/ml
• Specificity: Total Human IgG (Does not distinguish IgG subclasses.)
• Range: 0.2-200 ng/ml
• Sample Type: Serum, plasma, hybridoma cell supernatants, ascites or other biological fluids.
• Cross-Reactivity: Pooled normal plasma from Cynomolgus monkey was assayed and minor cross-reactivity was observed. Pooled normal plasma from mouse, rat and rabbit were assayed and no significant cross-reactivity was observed.

Reagents provided:

• 96-well microtiter strip plate(s) coated with anti- Human IgG antibody, blocked and dried on well surface
• Wash Buffer Concentrate (10x)
• Human IgG standard, lyophilized
• Anti-Human IgG primary antibody, lyophilized polyclonal antibody
• Horseradish peroxidase-conjugated Streptavidin, concentrated
• TMB substrate solution

CS222

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.