IGF-I

IGF-I, Mechano growth factor, Somatomedin-C

Human

CKH152A pdf (datasheet)

1 Plate

$650.00 BUY

The Human IGF-I ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGF-I in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and Biotinylated Anti-Human IGF-I antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Specifications:

• Specificity: Human IGF-I free, not complexed
• Sensitivity: 100 pg/ml
• Range: 0.123 ng/ml to 100 ng/ml
• Sample Type: Cell supernatants, serum, plasma samples.

Reagents and Material supplied in the kit include:

• Anti-Human IGF-I Microplate: 12 strips x 8 wells 96 wells
• Wash Buffer Concentrate (20x)
• Recombinant Human IGF-I Standard
• Assay Diluent C: Standard/Sample 
• Detection Antibody: Biotinylated Anti-Human IGF-I
• Streptavidin-HRP Concentrate (120x)
• TMB One-Step Substrate Reagent
• Stop Solution

IGF1

Insulin-like growth factor I

P05019

CKH152

Stable unopened for 6 months from date of shipment at 2-8°C or 1 year at -20°C. For extended stability, store at -80°C. Once opened, microplate strips and reagents are stable for 1 month at 2-8°C. Return unused strips to the pouch containing desiccant pack and reseal along the entire edge. Avoid repeated freeze-thaw cycles.

ELISA