IgE

Human

CS221A pdf (datasheet)

1 x 96 tests

$840.00 BUY

Background: IgE is the least abundant immunoglobulin in serum and is predominately involved in the allergy response. IgE binds to allergens and triggers histamine release from mast cells and basophils. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections.

Assay Principle: Human IgE will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgE antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human IgE. Color development is proportional to the concentration of IgE in the samples.

Specifications:

• Sensitivity: 0.38 ng/ml
• Specificity: Total Human IgE
• Range: 0.5-500 ng/ml
• Sample Type: Serum, plasma, cell culture supernatant, ascites or other biological fluids.
• Cross-Reactivity: Pooled normal plasma from mouse, rat, dog, horse, and sheep were assayed and no significant cross-reactivity was observed.

Reagents provided:

• 96-well microtiter strip plate(s) coated with anti-Human IgM antibody, blocked and dried on well surface
• Wash Buffer Concentrate (10x)
• Human IgE standard, lyophilized
• Anti-Human IgE horseradish peroxidase antibody, lyophilized polyclonal antibody
• TMB substrate solution

CS221

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.

ELISA