IgA

Human

CS163A pdf (datasheet)

1 x 96 tests

$710.00 BUY

Background: IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma. IgA in serum is a primarily monomeric 160kDa glycoprotein that initiates defenses against natural infection through interaction with specific receptors and immune mediators. Each monomer consists of two heavy chains and two kappa or lambda light chains. Most serum IgA molecules are subclass IgA1 which have longer hinge regions than subclass IgA2

Assay Principle: Human IgA will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgA antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human IgA. Color development is proportional to the concentration of IgA in the samples..

Specifications:

• Sensitivity: 0.035 ng/ml
• Specificity: Total Human IgA
• Range: 0.1-100 ng/ml
• Sample Type: Serum, plasma, cell culture supernatant, ascites or other biological fluids.

Reagents provided:

• 96-well microtiter strip plate(s) coated with anti-Human IgM antibody, blocked and dried on well surface
• Wash Buffer Concentrate (10x)
• Human IgA standard, lyophilized
• Anti-Human IgA horseradish peroxidase antibody, lyophilized polyclonal antibody
• TMB substrate solution

CS163

All kit components must be stored at 2-8°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 2-8°C. Kit should be used no later than the expiration date.

ELISA