Human Anti-SARS-CoV-2 Spike-RBD (S309) Neutralizing mAb

Synonyms:

Spike glycoprotein, Spike receptor binding domain, Spike-RB protein, Sotrovimab precursor, S309

Species:

SARS-CoV-2

Cat. No.:

CPC525B pdf (datasheet)

Quantity/Size:

100 µg

Price:

Please Inquire

Concentration:

1.0 mg/ml

Description:

New catalog number DVV00307

S309, which was first isolated from memory B cells from the sera of a recovered SARS-CoV-1 patient. S309, like many other antibodies, targets the Spike protein of the viral genome, which modulates viral entry into host cells and carries several antibody binding sites. S309 targets a specific residue on the Spike protein, N343, which was later determined to be a consistently conserved glycan in the Sarbecovirus subgenus. As SARS-CoV-2 belongs to this subgenus and maintains many similarities to SARS-CoV-1, S309 was a promising neutralizing antibody candidate for inhibition of SARS-CoV-2.

Uniprot Name:

Spike glycoprotein

Uniprot ID:

P0DTC2

Specificity:

Recognizes an epitope containing a glycan that is conserved within the Sarbecovirus subgenus, without competing with receptor attachment. Potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-

Host Species:

Human

Isotype:

IgG1

Clone:

S309

Formulation:

1.0 mg/ml, lot specific

Purification:

Protein A affinity chromatography

Application:

ELISA, Neutralizing , WB

Application notes:

ELISA: 1:5,000 - 1:10,000
Western blot: suggested dilution 1:1,000 - 1:2,000

This antibody may be used as the detecting Ab when paired with CPC527 as the capture antibody in a sandwich ELISA.

Storage/Stability:

Stable at 2-8°C for 1 week or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.