Human Anti-SARS-CoV-2 Spike S1 (CR3022) Neutralizing mAb

Synonyms:

Spike glycoprotein, Spike S1 subunit, S glycoprotein, CR3022

Species:

SARS-CoV-2

Cat. No.:

CPC520A pdf (datasheet)

Quantity/Size:

50 µg

Price:

Please Inquire

Concentration:

1.0 mg/ml

Description:

New catalog number RVV00303

CR3022, which was previously isolated from a convalescent SARS-CoV–infected patient, is a neutralizing antibody obtained in complex with the receptor-binding domain of the SARS-CoV-2 spike. The antibody binds to an epitope conserved between SARS-CoV-2 and SARS-CoV that is distinct from the receptor-binding site. CR3022 was shown to neutralize SARS-COV in a concerted action with clone CR3014. Presence of both antibodies delivers a blocking action of the SARS-COV RBD-ACE2 interaction, by binding two distinct and functional epitopes.

Uniprot Name:

Spike glycoprotein

Uniprot ID:

P0DTC2

Specificity:

Reacts with aa 318-510 in the S1 domain of the SARS-CoV and CoV-2 Spike protein. Binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein.

Host Species:

Human

Immunogen:

Isolated from a convalescent SARS-CoV–infected patient

Isotype:

IgG1

Clone:

CR3022

Formulation:

Sterile-filtered PBS, pH 7.5 preservative free.

Purification:

Protein A affinity chromatography

Application:

ELISA, FS , Neutralizing , WB

Application notes:

ELISA: 1:5,000 - 1:10,000
Western blot: suggested dilution 1:1,000 - 1:2,000

Storage/Stability:

Stable at 2-8°C for 1 week or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.