Angiogenin

Ribonuclease 5, RNase 5

Human

CK400 pdf (datasheet)

1 Plate

$595.00 BUY

The Human Angiogenin ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human Angiogenin antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Specifications:

Specificity: Human Angiogenin

Sensitivity: 1.5 pg/ml

Range: 1.64 pg/ml to 50 ng/ml

Sample Type: Cell supernatants, serum, plasma samples.

Reagents and Material supplied in the kit include:

• Anti-Human Angiogenin Microplate: 12 strips x 8 wells 96 wells
• Wash Buffer Concentrate (20x)
• Recombinant Human Angiogenin Standard
• Assay Diluent A: Standard/Sample – Plasma/Serum
• Assay Diluent B (5x): Standard/Sample - Cell Culture Medium
• Detection Antibody: Biotinylated Anti-Human Angiogeni
• Streptavidin-HRP Concentrate (700x)
• TMB One-Step Substrate Reagent
• Stop Solution

ANG

Angiogenin

P03950

CK400

Stable unopened for 6 months from date of shipment at 2-8°C or 1 year at -20°C. For extended stability, store at -80°C. Once opened, microplate strips and reagents are stable for 1 month at 2-8°C. Return unused strips to the pouch containing desiccant pack and reseal along the entire edge. Avoid repeated freeze-thaw cycles.

ELISA